ftir microspectroscopy reveals chemical changes in mice fetus following phenobarbital administration

نویسندگان

azadeh ashtarinezhad department of toxicology, school of pharmacy, shahidbeheshti university of medical sciences, tehran, iran

ataollah panahyab young researchers club, science and research branch, islamic azad university, tehran, iran.

baharak mohamadzadehasl department of toxicology, school of pharmacy, shahidbeheshti university of medical sciences, tehran, iran

hossein vatanpour pharmaceutical sciences research center, shahidbeheshti university of medical sciences, tehran, iran.

چکیده

phenobarbital is a phenobarbiturate used as a sedative, anticonvulsant, or hypnotic with the doses prescribed and can cause teratogenic effects. the goal of this study was to examine an alternative method for the recognition of the mechanism or the bimolecular potential changes in mice fetus caused by phenobarbital using ftir micro spectroscopy. the mice were injected with phenobarbital (120mg/kg) on gestation day 9. fetuses were dissected on day 15 of gestation and morphological and histological studies on the fetus were carried out. sections (10µm) of normal and phenobarbital treated fetus brains and livers were used for ftir measurement in the wave number region of 400- 4000 cm-1. the results were shown by 2ndderivativization of spectra & also subtraction from control spectra. in liver, the intensity at 1054 cm-1, 1155 cm-1, 1353 cm-1, 1453cm-1,1645 cm-1, 1622 cm-1, 2944 cm-1, 2913 cm-1 and 2845 cm-1 were shifted and increased. in the brain, the intensity at 879 cm-1, 911 cm-1, 955 cm-1, 1223 cm-1, 1256 cm-1, 1304 cm-1, 1360 cm-1, 1453 cm-1, 1529 cm-1, 1636 cm-1, 2845 cm-1, 2915 cm-1& 2950 cm-1 were increased and shifted. the most important changes of the fetus brain tissue are on the β structure of proteins due to the amide i bands at 1636 cm-1, while extensive effects on the dna structure were obvious for the phenobarbital treated liver tissues. as a conclusion, ftir spectroscopy might well be assumed as a potentially powerful teratogenic measurement instrument with a unique ability to identify the modified bimolecular structures.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

FTIR Microspectroscopy Reveals Chemical Changes in Mice Fetus Following Phenobarbital Administration

Phenobarbital is a phenobarbiturate used as a sedative, anticonvulsant, or hypnotic with the doses prescribed and can cause teratogenic effects. The goal of this study was to examine an alternative method for the recognition of the mechanism or the bimolecular potential changes in mice fetus caused by Phenobarbital using FTIR micro spectroscopy. The mice were injected with Phenobarbital (120mg/...

متن کامل

FTIR Microspectroscopy Reveals Chemical Changes in Mice Fetus Following Phenobarbital Administration

Phenobarbital is a phenobarbiturate used as a sedative, anticonvulsant, or hypnotic with the doses prescribed and can cause teratogenic effects. The goal of this study was to examine an alternative method for the recognition of the mechanism or the bimolecular potential changes in mice fetus caused by Phenobarbital using FTIR micro spectroscopy. The mice were injected with Phenobarbital (120mg/...

متن کامل

FTIR Microspectroscopy Reveals Chemical Changes in Mice Fetus Following Phenobarbital Administration

Phenobarbital is a phenobarbiturate used as a sedative, anticonvulsant or hypnotic with the doses prescribed and can cause teratogenic effects. The goal of this study was to examine an alternative method for the recognition of the mechanism or the bimolecular potential changes in mice fetus caused by Phenobarbital using FTIR micro spectroscopy. The mice were injected with Phenobarbital (120 mg/...

متن کامل

FTIR-Microspectroscopy Detection of Metronidazole Teratogenic Effects on Mice Fetus

Metronidazole is used to treat trichomoniasis, bacterial vaginosis, and other diseases. There are controversy aspects about its teratogenicity. A teratogenic agent can alter morphology or subsequent function of the fetus. The aim of this study was to examine an alternative method for the recognition of the mechanism or the bimolecular potential changes in mice fetus caused by Metronidazole usin...

متن کامل

FTIR-Microspectroscopy Detection of Metronidazole Teratogenic Effects on Mice Fetus

Metronidazole is used to treat trichomoniasis, bacterial vaginosis, and other diseases. There are controversy aspects about its teratogenicity. A teratogenic agent can alter morphology or subsequent function of the fetus. The aim of this study was to examine an alternative method for the recognition of the mechanism or the bimolecular potential changes in mice fetus caused by Metronidazole usin...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of pharmaceutical research

جلد ۱۴، شماره supplement، صفحات ۱۲۱-۱۳۰

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023